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| Registros recuperados: 174 | |
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| Shi,Mei; Zhou,Yaping; Cao,Limin; Ding,Cuijun; Ji,Yun; Jiang,Qinbo; Liu,Xiping; Li,Xiang; Hou,Xueling; Peng,Hongjun; Shi,Weifeng. |
| The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterovirus 71; Gene cloning; Recombinant VPl protein; ELISA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400027 |
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| Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan. |
| Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were... |
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Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995 |
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| Sanches, Simone Camargo; Carvalho, Cleber Eduardo Galvão; Fragoso, Stenio Perdigão; dos Santos, Lenita Ramires; Rosinha, Grácia Maria Soares; Soares, Cleber Oliveira. |
| O objetivo deste estudo foi desenvolver uma vacina de DNA contra linfadenite caseosa, testá-la em camundongos BALB/c e avaliar a produção de anticorpos específicos e os níveis de proteção conferidos aos animais testados. Para isso, o gene denominado proteína de superfície de membrana (psm), que produz uma proteína potencialmente antigênica, selecionado por técnica de imunovarredura de uma biblioteca de expressão de C. pseudotuberculosis, foi usado como alvo da construção de uma vacina de DNA. Parte desse gene foi amplificada pela PCR e clonada nos plasmídeos pET28a e pcDNA3.1+ para produção de proteína recombinante in vitro e expressão in vivo, respectivamente. Grupos de cinco camundongos da linhagem BALB/c receberam quatro doses do candidato a imunógeno e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Linfadenite Caseosa; Vacina de DNA; Proteína recombinante; ELISA. |
| Ano: 2015 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/24831 |
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| Souza,N.F.; Larsson,M.H.M.A. |
| The frequency of canine heartworm disease was determined in 310 dogs (158 males and 152 females) from São Paulo city (154) and several towns along the São Paulo state coast (156). The survey was carried out through the Knott's technique (direct presence of microfilariae in the blood) and ELISA for soluble circulating antigen detection. Twenty five (8.0%) samples were positive to D. immitis using both methods being 6 dogs (3.9%) from the city and 19 (12.2%) from the coastal area. Nineteen dogs (6.1%) were positive to the Knott's technique (4 from the city and 15 from the coastal area) and 12 dogs (3.9%) to the ELISA (4 from the city and 8 from the coastal area); six dogs (1.9%) were positive for both methods being two from the city and four from the coastal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Canine heartworm disease; Dirofilaria immitis; ELISA; Frequency. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300009 |
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| Royer, J; Seguineau, Catherine; Park, K; Pouvreau, Stephane; Choi, K; Costil, K. |
| Two methods were used to estimate the reproductive output of female Pacific oysters reared in Normandy: histology with image analysis and ELISA (Enzyme-Linked lmmunoSorbent Assay) which allowed the quantification of egg protein. Condition indices, gonad area and gametogenetic stages of the oysters were determined in the entire population (males and females) between May and October 2005. All investigations were performed in 3 age classes: oysters in their first, second or third years (corresponding to spat, half-grown and market-sized oysters, respectively). Both quantitative histology and ELISA provided similar results in terms of reproductive effort (illustrated by the gonado-somatic index, GSI) except during the GSI drop, corresponding to spawning, which... |
| Tipo: Text |
Palavras-chave: Reproductive effort; Image analysis; Gametogenetic cycle; ELISA; Crassostrea gigas; Condition index. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4305.pdf |
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| Vrat Kamboj,Dev; Nema,Vijay; Kumar Pandey,Arun; Kumar Goel,Ajay; Singh,Lokendra. |
| Staphylococcal food poisoning (SFP) is caused by the members of superantigen family called staphylococcal enterotoxins (SEs). About 20 different types of SEs are produced by Staphylococcus aureus out of which type A (SEA), B (SEB), C (SEC) and D (SED) are commonly implicated in SFP. Among these, SEB is the most potent toxin and has also gained the status of biological warfare (BW) agent. Therefore, detection of SEB is of utmost importance. Any immunological detection system for SEB requires specific and sensitive antibodies which inturn depends on the purity of the SEB. In the present investigation, seb gene of S. aureus was cloned and expressed in E. coli along with biotin as fusion partner to facilitate the purification process. The yield of purified... |
| Tipo: Journal article |
Palavras-chave: Affinity purification; Biotin fusion; ELISA; Gene cloning; SEB; Western blot. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500010 |
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| Barbosa,Celso Guimarães; Sanavria,Argemiro; Freire,Ronald Bastos. |
| Six bovines were infested with 60 first instar larvae of Dermatobia hominis. The animals were bleed weekly, and their antibodies levels to D. hominis L1, L2 and L3 instars measured during the time, following the infestation course. The antisera were submitted to a titration against optimal dilutions of antigen coated wells of microplates, previously sensitized with L1, L2 and L3 preparations, respectively. The ELISA assay was used to test single dilutions of antisera, which results were comparatively analyzed with a control of not infested animals. Antibodies against L1 were detected between the first and 21st day post-infestation (DPI) and, from the 42nd DPI on. Anti-L2 antibodies, could be detected on the 21st DPI and from the 35th DPI until... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dermatobia hominis; Myiasis; Humoral immune response; ELISA. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000300013 |
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| Sepúlveda R,Paulina; Larraín S,Patricia; Quiroz E,Carlos; Rebufel A,Patricia; Graña S,Fernando. |
| Durante las temporadas de 2001-2002 y 2002-2003 se realizó una prospección de virus en pimientos (Capsicum annuum L.) en diversas localidades de la Región de Coquimbo (29,5-32° lat. Sur). La determinación de virus se realizó mediante la prueba DAS- ELISA. La importancia relativa de los virus encontrados para ambas temporadas fueron: Virus del mosaico del pepino (CMV) 23,3%; Virus del bronceado del tomate (TSWV) 20,8%; Virus del mosaico de la alfalfa (AMV) 14,8%;Virus Y de la papa (PVY) 14,5% e Impatients necrotic spot virus (INSV) 3,1% (primera identificación de este virus en pimiento en Chile); Virus del mosaico del tomate (ToMV) 2,2% y Virus del mosaico del tabaco (TMV) 4,9%. Los resultados indicaron que al menos el 30% de las muestras estaba infectada... |
| Tipo: Journal article |
Palavras-chave: Virus en pimiento; Insectos vectores; Tomato spotted wilt virus; Cucumber mosaic virus; Alfalfa mosaic virus; Potato virus Y; ELISA. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072005000300001 |
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| ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇. |
| Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione... |
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Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983 |
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| MASATANI, Tatsunori; OOKA, Hideo; TERKAWI, Mohamad A.; CAO, Shinuo; LUO, Yuzi; ASADA, Masahito; HAYASHI, Kei; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄, 学南. |
| Babesia microti is a rodent tick-borne blood parasite and the major causative agent of emerging human babesiosis. Here, we identified a candidate of common antigenic protein BmP41 of B. microti by serological screening of cDNA library of human-pathogenic Gray strain with antisera against rodent Munich strain. Immunofluorescent antibody test using mouse anti-recombinant BmP41 (rBmP41) serum revealed that native BmP41 was expressed in each of the developmental stages of B. microti merozoites. An enzyme-linked immunosorbent assay (ELISA) using rBmP41 detected specific antibodies in sera from hamsters infected with B. microti Gray strain and mice infected with B. microti Munich strain. Taken together, BmP41 could be a promising universal serological marker for... |
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Palavras-chave: Babesia microti; BmP41; Common antigen; ELISA; Immunoscreening. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3917 |
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| Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.. |
| ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hyperimmunization; Irradiation; Crotalus durissus terrificus; ELISA; Serum neutralization; Detoxification. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008 |
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| Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.. |
| ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venom; Irradiation; Bothrops; ELISA; Serum production; Detoxification. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400006 |
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| Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.. |
| Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004 |
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| Soares,K.A.; Silva,M.R.M.; Poleti,M.D.; Maia,A.A.M.. |
| Colheu-se sangue de sete suínos infectados com ovos de Taenia solium, semanalmente, durante 140 dias, para realizar ELISA no soro, utilizando antígeno de escólex (Es-Tso) de C. cellulosae. Em todos os animais, após o 21º dia pós-infecção, houve incremento significativo de anticorpos IgG, que assim se mantiveram até o final do experimento. A sensibilidade do ELISA variou entre 87,5 e 100%. À necropsia, foram identificados 238 cisticercos. Seis suínos apresentaram pelo menos um cisto no coração, língua ou masseter. Não se observou correlação entre concentração de anticorpos e número de cisticercos identificados. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Suíno; Cisticercose; Cysticercus cellulosae; Antígeno; ELISA. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000100003 |
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| Registros recuperados: 174 | |
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